Single Cell Analysis
We propose a novel handling technique of chemical substances termed ‘partipetting’, which allows the one-step pipetting of various chemical-laden hydrogels. We pipette and assemble various types of encoded chemical-laden microparticles in microwell arrays in parallel. The combination of this heterogeneous particle chip and a cell chip induces the release of the chemicals from the hydrogels and, eventually, the chemicals treat the targets.
Low Cost DNA synthesis
We propose ‘Sniper Cloning’ which enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for full utilization of huge number of clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system. It potentiates in serving as a universal tool for DNA writing in biological sciences.
By applying our Sniper clonging method, we can generate error-free DNA/RNA in high-throughput and low cost. This method also allows longer oligosynthesiscompared to conventional chemical synthesizing methods. With these high-quality templates, we aim to discovery new mechanism to generates self-assembled nucleic acid nano-structures or nano-robots, which could be used in target-specific drug delivery agent or nano manipulator in living cells.
Single Cell Analysis
Single-Cell Cancer Genomics
Current development of next generation sequencing (NGS) has raised many applications and one of them is SINGLE-CELL ANALYSIS. Many studies report that genome in every single-cell has similar but slightly different, which stands out especially in cancer cells. In this case, therapy method and survival rate for patient are affected by mutated genomic patterns. Our project aims to develop single-cell isolation technology for comparing genomic sequences in single-cell level.
Drug Susceptibility Test
The conventional Drug Susceptibility Test (DST) is based on naked eye detection and takes about 4~6weeks to obtain DST results. In comparison, our Disk Agarose Channel (DAC) chip uses single cell tracking and provides accurate results in only 4 days. A treatment based on a rapid and accurate DST will improve outcomes for TB patients and reduce transmission of TB.
We generate massive genotype/phenotype matching data and screen Fc fragment variations. In detail, we optimize hinge-CH2 domain for desired effector function and CH2-CH3 domain for controlled antibody in vivo life-time. We also screen framework variation to development of super-ionized antibody for increased tissue/vessel penetration.
DNA-based Nano Construction
Rapid Drug Susceptibility Test
Antibiotic Susceptibility Test
We demonstrate a microfluidic agarose channel (MAC) system that reduces the AST assay time for determining MICs by single bacterial time lapse imaging. In single-cell tracking using a microscope, changes in bacterial cells can be detected as soon as cells divide, so antibiotic susceptibility can be determined in 3~4 hours compared to 16 hours when using conventional OD measurement. Rapid AST in microbiology laboratories can have a major impact on the care and outcome of hospitalized patients with infections that require curative antibiotic treatments.
We propose a novel drug screening platform based on a our new concept of liquid handling method called ‘partipetting’, which enable whole drug screening with a single pipetting process. To demonstrate our concept of liquid handling, we present a generation of liquid capped microsphere particles, heterogeneous assembly of different liquid capped particles, breaking and releasing of these liquid capped microshell particles.
Next Generation Diagnosis
Optofluidic Maskless Lithography(OFML)
Optofluidic maskless lithography is a technique that can synthesize free-floating microstructures in microfluidic channels at the desired time and location within the field of view of the lithography system. By uniquely combining the concept of maskless and continuous-flow lithography techniques in the microfluidic channels, we experimentally demonstrate real-time control of the in-situ polymerization process to dynamically synthesize extruded polymeric microstructures with various two-dimensional shapes. [Su Eun et al., Applied Physics Letters, Vol. 91, No. 4, p. 041106, 2007.]
We propose vivid, free-floating structural coloured particles with multi-axis rotational control using a colour-tunable magnetic material and a new printing method. Our colour-barcoded magnetic microparticles offer a coding capacity easily into the billions with distinct magnetic handling capabilities including active positioning for code readouts and active stirring for improved reaction kinetics in microscale environments. A DNA hybridization assay is done using the colour-barcoded magnetic microparticles to demonstrate multiplexing capabilities. [Howon Lee et al., Nature Materials, 9, 745-749, 2010]
“Railed microfluidics,” is a fully deterministic way of guiding microstructures inside a fluidic channel. Using railed microfluidics, we achieved fluidic self assembly of various complex heterogeneous microstructures. By transporting many different microstructures to exact assembly sites, complex 1D and 2D systems are assembled without wasting a single microstructure.